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Updated: Aug 3, 2026

Preparation and Immunofluorescence Staining of Bundles and Single Fiber Cells from the Cortex and Nucleus of the Eye Lens
Published on: June 9, 2023
Ocular lens gap junctions: protein expression, assembly, and structure-function analysis
J Kistler1, C Evans, P Donaldson
1Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Auckland, New Zealand.
Abstract:
Recent advances in understanding lens fiber gap junction formation are reviewed. These include studies of junctional protein expression in the embryonic lens, and of age related changes affecting gap junction structure and composition in the adult lens. An in vitro assembly system based on detergent solubilized pore complexes and endogenous lipids has been developed to provide information on the molecular interactions involved in gap junction formation and to provide material for structure analysis. Important information on the electrical properties of lens gap junction channels is obtained using electrophysiological techniques including planar lipid bilayer analysis and patch clamping.
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