Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Quantitative junctional permeability measurements using the confocal microscope

¹Department of Molecular and Cellular Physiology, Stanford, CA 94305, USA.

Microscopy Research and Technique

|August 1, 1995

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of dopamine on T-lymphocyte proliferative responses and serum prolactin concentrations in critically ill patients.

Critical care medicine·1992

Same author

Lung function testing: selection of reference values and interpretative strategies.

The American review of respiratory disease·1992

Same author

Defense behavior and coping in an autistic savant: the story of Temple Grandin, PhD.

Psychiatry·1992

Same author

Comparative quantitative evaluation of pleural fibrosis and its effects on pulmonary function in two large asbestos-exposed occupational groups--insulators and sheet metal workers.

Environmental research·1992

Same author

Identification of a repetitive element in the snail Biomphalaria glabrata: relationship to the reverse transcriptase-encoding sequence in LINE-1 transposons.

Gene·1992

Same author

On creativity.

Arthritis and rheumatism·1992

Same journal

Deep Learning Based Framework for Detection and Classification of Leukemia Using Microscopic Images.

Microscopy research and technique·2026

Same journal

Externally Controlled In Situ SEM: Multi-Rate Scanning With Signal Regulation and Spatiotemporal Fusion.

Microscopy research and technique·2026

Same journal

In Situ TEM Observation of Phase Transformation Nucleation at the Near-Surface of Synthetic Aragonite.

Microscopy research and technique·2026

Same journal

Morpho-Anatomical and HPTLC Investigations of Lysimachia nummularia L. (Primulaceae) Grown in Switzerland.

Microscopy research and technique·2026

Same journal

Macroscopic, Histological and Ultrastructural Features of the Tongue of the Anatolian Wild Boar (Sus scrofa libycus).

Microscopy research and technique·2026

Same journal

Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm, Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae).

Microscopy research and technique·2026

See all related articles

Cell-to-cell transfer of 5,6 carboxyfluorescein was compared in embryonic chick lens cells. Dissociated cells showed a higher transfer rate than cells in the intact anterior lens epithelium.

Area of Science:

Ophthalmology
Developmental Biology
Cell Biology

Background:

Cell-to-cell communication is crucial for tissue development and function.
Understanding gap junction-mediated communication in the developing lens is vital for eye development research.

Purpose of the Study:

To compare the rate of cell-to-cell transfer of a fluorescent tracer in dissociated embryonic chick lens cells versus cells within the intact anterior lens epithelium.
To investigate the impact of tissue structure on intercellular communication.

Main Methods:

Utilized a photobleach method combined with confocal microscopy.
Quantified the transfer rate of 5,6 carboxyfluorescein (a gap junction-permeant dye).
Compared transfer rates between isolated single cells and cells within the intact anterior lens epithelium of embryonic chick lenses.

Related Experiment Videos

Main Results:

The average cell-to-cell transfer rate in dissociated embryonic chick lens cells was 7.9 x 10^-3 sec^-1.
The average cell-to-cell transfer rate in the anterior epithelium of the intact embryonic chick lens was 2.6 x 10^-3 sec^-1.
Dissociated cells exhibited a significantly higher transfer rate compared to cells in the intact tissue.

Conclusions:

Tissue architecture in the embryonic chick lens significantly influences intercellular communication rates.
Dissociation of lens cells alters gap junction permeability or the effective diffusion distance.
These findings provide insights into the regulation of cell communication during ocular development.