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Sensitive chemiluminescence enzyme immunoassay for vascular endothelial growth factor/vascular permeability factor in
M Hanatani1, Y Tanaka, S Kondo
1Department of Bioscience Research, Tsukuba Research Laboratory, Toagosei Co., Ltd., Ibaraki, Japan.
Bioscience, Biotechnology, and Biochemistry
|October 1, 1995
Abstract:
A sandwich chemiluminescence enzyme immunoassay for measuring the level of VEGF/VPF in serum was constructed. The detectability of the assay is very low (1.0 pg/ml) and the measurable range of the assay was very wide (1-1000 pg/ml). The assay showed that the average level of VEGF/VPF in human sera from healthy blood donors was approximately 19 pg/ml.