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Determination of the structure of the exopolysaccharide produced by Lactobacillus sake 0-1
G W Robijn1, D J van den Berg, H Haas
1Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Carbohydrate Research
|October 16, 1995
Abstract:
The exopolysaccharide produced by Lactobacillus sake 0-1 in a semi-defined medium was found to have an average molecular mass of 6 x 10(6) Da and a composition of D-glucose, L-rhamnose, and sn-glycerol 3-phosphate (3:2:1). The polysaccharide is partially O-acetylated. By means of partial acid hydrolysis, O-deacetylation, deglycerophosphorylation, methylation analysis, and 1D/2D NMR (1H, 13C, and 31P) studies the polysaccharide was shown to be composed of repeating units with the following structure: [formula: see text]