Related Experiment Videos
Effect of intraperitoneally administered agents on human peritoneal mesothelial cell growth
1Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, ROC.
Abstract:
During continuous ambulatory peritoneal dialysis, the peritoneal mesothelial cell layer is under continuous sloughing and regeneration processes. Agents unfavorable for mesothelial cell growth may be harmful to the peritoneal membrane. We investigated whether frequent intraperitoneally instilled agents affect mesothelial cell growth. Peritoneal mesothelial cells were cultured from the human omentum. The proliferation was assessed by using a modified methyltetrazolium assay and confirmed by Coulter cell counting. The results showed that a high-glucose medium and heparin inhibited mesothelial cell growth. Cephalothin at the usual intraperitoneal loading and maintenance doses is toxic to mesothelial cells. Ceftazidime is toxic to mesothelial cells at its loading dose and inhibits growth at its maintenance dose. Aminoglycosides including netilmicin, gentamicin, and amikacin all had inhibitory effects at the loading and maintenance dose ranges. Vancomycin had no effect. The usual combinations of heparin and cephalothin with netilmicin or gentamicin as the initial treatment regimen for bacterial peritonitis are toxic to mesothelial cells. These results suggest that some intraperitoneal agents potentially may hamper mesothelial cell regeneration. The judicious use of heparin and the proper choice of antibiotic combinations may be warranted from the point of view of peritoneal protection.
Insights
Certain medications used in peritoneal dialysis, like some antibiotics and heparin, can harm the cells crucial for peritoneal membrane health. Careful selection of these agents is vital for protecting the peritoneal membrane during dialysis.
Area of Science:
- Nephrology
- Cell Biology
- Peritoneal Dialysis
Background:
- The peritoneal membrane's mesothelial cells undergo constant renewal during peritoneal dialysis.
- Agents detrimental to mesothelial cell proliferation may compromise peritoneal membrane integrity.
Purpose of the Study:
- To evaluate the impact of frequently instilled intraperitoneal agents on human peritoneal mesothelial cell growth.
- To identify specific agents and combinations that may hinder mesothelial cell regeneration.
Main Methods:
- Human omental peritoneal mesothelial cells were cultured in vitro.
- Cell proliferation was quantified using a modified methyltetrazolium assay and Coulter cell counting.
Main Results:
- High-glucose medium and heparin demonstrated inhibitory effects on mesothelial cell growth.
- Cephalothin was toxic at standard doses; ceftazidime showed toxicity at loading doses and inhibition at maintenance doses.
- Aminoglycosides (netilmicin, gentamicin, amikacin) inhibited growth at typical doses, while vancomycin had no significant effect.
- Common combinations of heparin and antibiotics (cephalothin with netilmicin or gentamicin) proved toxic to mesothelial cells.
Conclusions:
- Several commonly used intraperitoneal agents, particularly certain antibiotics and heparin, can negatively affect mesothelial cell growth and regeneration.
- Judicious use of heparin and strategic selection of antibiotic combinations are recommended for preserving peritoneal membrane health during dialysis.