[The organ culture of rabbit bladder epithelium using a perfluorocarbon emulsion]

The influence of perfluorocarbon emulsion on the development of the growth zone in the organ culture of rabbit bladder epithelium was studied. The results of histological investigations suggest that the preliminary treatment of epithelium explants with perfluorocarbon emulsion for 4 h not only preserves the explants' viability, but enhances the growth zone development. The treatment of explants with perfluorocarbon emulsion before deep freezing increases the cryoresistance of tissue explants and enhances development of the growth zone. The data obtained indicate that perfluorocarbon emulsion can be used for both the storage of tissue explants at +4 degrees C and their cryoconservation.