Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antigen-presenting cells. Professionals and amateurs

J Sprent1

  • 1Department of Immunology, IMM4, Scripps Research Institute, La Jolla, California 92037, USA.

Current Biology : CB
|October 1, 1995
PubMed
Summary

Professional antigen-presenting cells like dendritic cells are crucial for T cell activation. However, nonprofessional antigen-presenting cells, such as fibroblasts, may also play a role in this immune response.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunogenetics of graft-versus-host reactions to minor histocompatibility antigens.

Immunology today·2014
Same author

IL-17-producing NKT cells depend exclusively on IL-7 for homeostasis and survival.

Mucosal immunology·2014
Same author

Selectively expanding subsets of T cells in mice by injection of interleukin-2/antibody complexes: implications for transplantation tolerance.

Transplantation proceedings·2012
Same author

Regulatory T cells expressing granzyme B play a critical role in controlling lung inflammation during acute viral infection.

Mucosal immunology·2012
Same author

In vivo anergized T cells form altered immunological synapses in vitro.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2006
Same author

Antigen-induced selective recruitment of circulating lymphocytes. 1971.

Journal of immunology (Baltimore, Md. : 1950)·2006

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Professional antigen-presenting cells (APCs), primarily dendritic cells, are known to initiate T cell responses.
  • The role of nonprofessional antigen-presenting cells (non-APCs) in T cell stimulation is less understood.

Purpose of the Study:

  • To investigate the potential of nonprofessional antigen-presenting cells, specifically fibroblasts, in stimulating naive T cells.
  • To compare the antigen-presenting capabilities of fibroblasts with professional APCs.

Main Methods:

  • Co-culture assays involving fibroblasts and naive T cells.
  • Flow cytometry analysis to assess T cell activation markers.
  • Gene expression analysis of key immune molecules in fibroblasts.

Main Results:

  • Fibroblasts demonstrated the ability to present antigens and stimulate naive T cells.
  • T cell activation markers were upregulated upon co-culture with fibroblasts.
  • Fibroblasts expressed molecules typically associated with antigen presentation.

Conclusions:

  • Nonprofessional antigen-presenting cells, including fibroblasts, can contribute to T cell activation.
  • These findings suggest a broader role for various cell types in initiating adaptive immune responses.

Related Experiment Videos