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Receptor-mediated induction of human glomerular epithelial cell alkaline phosphodiesterase I by glucocorticoids
V Stefanović1, V Djordjević, M Mitić
1Institute of Nephrology and Hemodialysis, Faculty of Medicine, Nis, Yugoslavia.
Abstract:
Alkaline phosphodiesterase I was demonstrated in human glomerular mesangial cells (HGEC) as an ectoenzyme. Treatment of HGEC by dexamethasone increased surface phosphodiesterase I activity in a dose- and time-dependent manner. Maximal increase of phosphodiesterase I activity, about twice, occurred after treatment with 5 microM dexamethasone for 6 days. Cycloheximide prevented and RU 38486, a glucocorticoid receptor antagonist, suppressed the dexamethasone induced increase in phosphodiesterase I activity. This study shows that HGEC have a surface phosphodiesterase I controlled by glucocorticoids through a receptor-mediated mechanism.
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