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Related Experiment Videos

A theoretical analysis of intracellular oxygen diffusion

A Dutta1, A S Popel

  • 1Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.

Journal of Theoretical Biology
|October 21, 1995
PubMed
Summary

Cellular oxygen diffusion is heterogeneous, influenced by lipid membranes acting as pathways. Muscle tissue oxygen permeability varies significantly with lipid content, requiring caution when extrapolating data between different muscle types.

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Area of Science:

  • Biophysics
  • Cellular Physiology
  • Membrane Transport

Background:

  • Oxygen diffusion within cells is critical for cellular respiration.
  • Intracellular oxygen transport may be heterogeneous, influenced by cellular structures like mitochondria and lipid membranes.
  • Previous models often simplified oxygen diffusion pathways, potentially underestimating tissue permeability.

Purpose of the Study:

  • To investigate the role of intracellular pathways, particularly lipid membranes, in oxygen diffusion.
  • To determine the temperature-dependent permeability of cytosol and lipids within muscle tissue.
  • To compare calculated intracellular permeabilities with experimental data and assess variability across different muscle types.

Main Methods:

  • Analysis of experimental tissue oxygen permeability data using theoretical models of diffusion in two-phase media.

Related Experiment Videos

  • Modeling muscle tissue as a combination of aqueous cytosol and lipids.
  • Determination of cytosolic and lipid permeability as a function of temperature.
  • Comparison of model-derived permeabilities with in vitro and reported experimental values.

    • Main Results:

    • Cytosolic permeability varied from 50% to 90% of water permeability with temperature, showing a different dependence than water.
    • A parallel model of lipid and cytosol yielded lipid permeability 71% higher than in vitro values.
    • Calculated intracellular permeabilities were inconsistent with reported values for rat cardiac myocytes.
    • Higher lipid content correlated with increased tissue oxygen permeability across three different muscles.

    Conclusions:

    • Muscle tissue oxygen permeability is influenced by both lipid and cytosol components and varies significantly between muscle types.
    • Lipid membranes likely act as important oxygen diffusion pathways.
    • Extrapolation of oxygen permeability data between different muscle types should be done cautiously.
    • Oxidative muscles with high lipid content may have higher oxygen permeability than commonly assumed, warranting further investigation at physiological temperatures.