[Antihemolytic effect of chlorpromazine on erythrocytes in hyperosmotic and cold shock]

The combined effect of preliminary dehydration of erythrocytes and the cationic amphiphilic compound, chlorpromazine, on the stability of cells to subsequent hyperosmotic effect, has been studied. A protective effect of chlorpromazine depending on the degree of initial cell dehydration and temperature was found. The results obtained by an addition of the cationic amphiphile at preliminary stages of the experiment and directly in the process of development of hyperosmotic lysis have been compared. A conclusion was drawn that the observed effect is not due to preliminary treatment of cells with chlorpromazine and is therefore unrelated to its influence on the regulatory systems of the cell. It has been shown also that under cold shock conditions the earlier established protective effect of chlorpromazine is also directly realized at the moment of stress exposure, i.e., with a change in temperature.