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Endothelial cell binding to Dacron modified with polyethylene oxide and peptide
D B Holt1, R C Eberhart, M D Prager
1Department of Surgery, University of Texas Southwestern Medical Center, Dallas 75235-9031, USA.
ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|July 1, 1994
Summary
Researchers developed a new surface for Dacron vascular prostheses by incorporating polyethylene oxide (PEO) and an endothelial cell (EC) adhesion peptide. This modification selectively enhances EC adherence while reducing fibroblast binding, improving biocompatibility for vascular grafts.
Area of Science:
- Biomaterials Science
- Vascular Prosthetics
- Surface Chemistry
Background:
- Dacron (PET) vascular prosthetics lack ideal endothelial cell (EC) integration.
- Surface modification is crucial for improving the biocompatibility of vascular grafts.
- Selective cell adhesion is a key goal in designing advanced biomaterials.
Purpose of the Study:
- To develop a surface modification strategy for Dacron vascular prostheses.
- To achieve selective adherence of endothelial cells (ECs) to the prosthetic surface.
- To investigate the role of polyethylene oxide (PEO) and an EC adhesion peptide (GREDVY) in cell binding.
Main Methods:
- Incorporation of polyethylene oxide (PEO) onto Dacron (PET) material using trifluoroacetic acid (TFA).
- Covalent attachment of the Gly-Arg-Glu-Asp-Val-Tyr (GREDVY) peptide to the modified surface.
- Characterization using scanning electron microscopy, NMR, bromphenol blue staining, and tensile testing.
- Assessment of EC and human fibroblast binding to modified and unmodified surfaces.
Main Results:
- Optimal PEO incorporation was achieved using 78% TFA with minimal fiber damage.
- Low molecular weight PEO (1.5 kD) enhanced GREDVY peptide attachment and EC binding compared to high molecular weight PEO (18.5 kD).
- GREDVY modification consistently increased EC binding to PEO-modified Dacron.
- Human fibroblast binding decreased significantly upon GREDVY modification of the Dacron or PEO-modified Dacron surfaces.
Conclusions:
- Surface modification of Dacron vascular prostheses with PEO and GREDVY peptide enables selective endothelial cell adherence.
- The molecular weight of PEO plays a critical role in modulating cell adhesion.
- This approach holds promise for creating more biocompatible vascular grafts with improved integration.