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Related Experiment Videos

Mouse tetranectin: cDNA sequence, tissue-specific expression, and chromosomal mapping

K Ibaraki1, C A Kozak, U M Wewer

  • 1Bone Research Branch, National Institute of Dental Research National Institutes of Health (NIH), Bethesda, Maryland 20892, USA.

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|October 1, 1995
PubMed
Summary
This summary is machine-generated.

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Tetranectin gene regulation was studied by cloning mouse tetranectin cDNA. Sequence analysis revealed high homology to human tetranectin, with conserved structural elements suggesting functional importance.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Tetranectin is a plasminogen-binding protein with ubiquitous expression, notably high in tumor stroma and during mineralization.
  • Understanding tetranectin gene regulation is crucial for elucidating its biological roles.

Purpose of the Study:

  • To dissect the molecular basis of mouse tetranectin gene regulation.
  • To compare mouse and human tetranectin cDNA sequences and identify conserved structural features.
  • To determine the chromosomal location of the mouse tetranectin gene.

Main Methods:

  • Cloning of mouse tetranectin cDNA from an embryonic library.
  • Sequence analysis of cDNA and deduced amino acid sequences.
  • Northern blot analysis and RT-PCR (reverse transcription polymerase chain reaction) for transcript analysis.

Related Experiment Videos

  • Multilocus crosses for genetic mapping.

    • Main Results:

    • A 992-bp mouse tetranectin cDNA was identified with a 606-bp open reading frame, showing 76% amino acid identity and 87% similarity to human tetranectin.
    • Conserved cysteine positions and other residues indicate a crucial structure for tetranectin function.
    • Northern analysis revealed a major transcript of approximately 1 kb across species, with minor bands not confirmed as tetranectin products by RT-PCR.
    • The mouse tetranectin gene (Tna) was mapped to distal Chromosome 9.

    Conclusions:

    • Mouse and human tetranectin share significant sequence homology and conserved structural elements, implying conserved functions.
    • The primary tetranectin transcript is approximately 1 kb, and additional minor transcripts are unlikely to be gene products.
    • The genetic location of the tetranectin gene on mouse Chromosome 9 provides a basis for further genetic studies.