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Functional and genetic analysis of annexin VI
1Department of Physiology, University College London, UK.
Molecular and Cellular Biochemistry
|August 1, 1995
Summary
Annexin VI, a calcium-binding protein, undergoes growth-dependent modification affecting cancer cell growth. This post-translational modification is crucial for annexin VI
Area of Science:
- Molecular and Cellular Biology
- Biochemistry
- Cancer Research
Background:
- Annexin VI is a 68kDa calcium-binding protein.
- Its function and regulation are under investigation.
- Annexin VI is expressed heterologously in human A431 carcinoma cells.
Purpose of the Study:
- To determine the function of annexin VI.
- To analyze the structure and regulation of the annexin VI gene.
- To investigate a novel growth-dependent post-translational modification of annexin VI.
Main Methods:
- Heterologous expression of annexin VI in human A431 carcinoma cells.
- Analysis of gene structure and regulation.
- Investigation of post-translational modification and sub-cellular localization.
Main Results:
- Annexin VI exhibits a negative effect on A431 cells, partially reversing the transformed phenotype.
- A novel growth-dependent post-translational modification of annexin VI was discovered.
- The study explores the hypothesis that this modification is essential for sub-cellular targeting and function.
Conclusions:
- Post-translational modification of annexin VI plays a critical role in its cellular function.
- Proper sub-cellular localization, dependent on modification, is essential for annexin VI activity.
- Annexin VI may represent a target for modulating cancer cell phenotype.
