Related Experiment Videos
Chronic tympanic membrane perforation: an animal model
1Service d'Oto-Rhino-Laryngologie, de Chirurgie Cervico-Faciale et de Phoniatrie, Hôpital Edouard Herriot, Lyon, France.
The American Journal of Otology
|March 1, 1995
Summary
Developing a persistent tympanic membrane perforation model in dogs is crucial. Glutaraldehyde treatment created a more chronic perforation, unlike spontaneous healing, offering a better model for therapeutic research.
Area of Science:
- Veterinary Medicine
- Otolaryngology
- Biomaterials Science
Background:
- Acute tympanic membrane (TM) perforations in animals heal spontaneously, limiting their use as models for chronic perforations.
- Existing animal models do not accurately reflect the pathology of long-standing human TM perforations.
Purpose of the Study:
- To develop and validate a reliable animal model for persistent tympanic membrane perforation.
- To assess the efficacy of glutaraldehyde in creating a non-healing TM perforation model.
Main Methods:
- Bilateral subtotal symmetric perforations were created in 14 dogs.
- Right ears were treated with 2% glutaraldehyde to induce persistent perforation.
- Left ears served as controls with spontaneous healing.
- Healing was monitored weekly for 15 weeks, with histological analysis.
Main Results:
- Only 6 out of 14 glutaraldehyde-treated ears healed within 15 weeks, compared to 14 out of 14 control ears.
- Glutaraldehyde treatment significantly delayed TM perforation closure.
- Histological analysis confirmed persistent perforation in treated ears.
Conclusions:
- Glutaraldehyde treatment effectively creates a persistent tympanic membrane perforation model in dogs.
- This model is suitable for evaluating graft materials and healing-promoting substances for chronic TM perforations.