[Coccidioides immitis: isolation from soil samples in San Luis and Mendoza provinces]

By using the double pour plate method, Yeasts Extract Agar (YE) which contained a high concentration of chloramphenicol-streptomycin sulfate and cycloheximide,, and by inoculating mice intraperitoneally, we succeed in the isolation of different strains of Coccidicides immitis from 12 samples of the soils collected in the province of San Luis and from 1 of the province of Mendoza (Argentina), a 70% of the samples turned out positive. Identification of the strains was based on macro- and micro-morphology of the colonies, pathogenicity for mice and the capacity to transform in shaked cultures.