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Chemiluminescence in drug assay
A C Calokerinos1, N T Deftereos, W R Baeyens
1Laboratory of Analytical Chemistry, University of Athens, Greece.
Journal of Pharmaceutical and Biomedical Analysis
|August 1, 1995
Summary
Chemiluminescence enables simple, sensitive, and cost-effective analytical methods, especially for drug analysis when combined with chromatography. These techniques offer excellent sensitivity and computer control for streamlined laboratory procedures.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Chemiluminescence (CL) offers a pathway for developing analytical methods.
- These methods are characterized by simplicity, low instrumentation costs, and computer controllability.
- High sensitivity is a key feature of CL-based analyses.
Purpose of the Study:
- To review the applications of chemiluminescence in analytical procedures.
- To highlight the advantages of CL, including sensitivity and cost-effectiveness.
- To focus on the specific application of chemiluminometric methods in drug analysis.
Main Methods:
- Utilizing chemiluminescence phenomenon for analytical detection.
- Employing high-performance liquid chromatography (HPLC) for enhanced selectivity.
- Integrating computer control for instrumentation management.
Main Results:
- Chemiluminescence provides excellent sensitivity in analytical measurements.
- Combining CL with HPLC significantly improves the selectivity of methods.
- Computer control facilitates ease of operation for analytical instrumentation.
Conclusions:
- Chemiluminescence is a powerful tool for developing simple and sensitive analytical methods.
- The integration of HPLC enhances the specificity of chemiluminometric assays.
- Drug analysis is a significant and expanding application area for these techniques.