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Two-photon induced fluorescence of linear alkanes; a possible intrinsic lipid probe
I Gryczynski1, A Razynska, J R Lakowicz
1Department of Biological Chemistry, University of Maryland, School of Medicine, Baltimore 21201, USA.
Biophysical Chemistry
|January 1, 1996
Abstract:
We measured the fluorescence emission spectra and intensity decays of the linear alkane tetradecane when excited at 300 nm by two-photon excitation. The unquenched lifetime of tetradecane in neat solution is near 4.4 ns. The emission of tetradecane centered at 210 nm is collisionally quenched by oxygen, n-propanol and water. These results suggest that aliphatic groups in non-polar environments can display good fluorescence, and that the aliphatic side chains of detergents and lipids may serve as an intrinsic fluorescent probe of micelles and bilayers.