Capillary electrophoretic methods for a clear identification of selenoamino acids in complex matrices such as human...

Selenoamino acids from size-exclusionchromatographic fractions of human milk were identified and determined by capillary electrophoresis (CE). For this purpose, different CE methods were developed to separate these selenoamino acids from other molecules with similar molecular masses. Methods were introduced for the clear identification of the analytes. These methods were designed to allow the identification of selenoamino acids in spite of the presence of many molecules with similar mobilities. Further, they overcome identification problems caused by shifts of migration times (due to different ionic compositions) compared with standard solutions.