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T-vector cloning and high performance PCR with SuperTth from Thermus thermophilus
J F Leal1, J López-Barea, G Dorado
1Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Córdoba, Spain.
Genetic Analysis : Biomolecular Engineering
|October 1, 1995
Abstract:
The SuperTth DNA polymerase from Thermus thermophilus exhibits template-independent terminal transferase (extendase) activity. This enzyme is proposed as a cheap alternative for both high performance PCR as well as quick T-vector cloning of amplicons, including reverse transcription and cDNA cloning.