Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure of the elastin gene

J Rosenbloom1, W R Abrams, Z Indik

  • 1Department of Anatomy and Histology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104, USA.

Ciba Foundation Symposium
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Case Report about Glycogenic Hepatopathy.

Case reports in endocrinology·2022
Same author

Structural and Functional Characteristics of the Microbiome in Deep-Dentin Caries.

Journal of dental research·2020
Same author

Simulating Tsunami Inundation and Soil Response in a Large Centrifuge.

Scientific reports·2019
Same author

Regulatory B Cell-Dependent Islet Transplant Tolerance Is Also Natural Killer Cell Dependent.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
Same author

The Effects of Exogenous Administration of Human Coagulation Factors Following Pig-to-Baboon Liver Xenotransplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

Pilot Study Evaluating Regulatory T Cell-Promoting Immunosuppression and Nonimmunogenic Donor Antigen Delivery in a Nonhuman Primate Islet Allotransplantation Model.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same journal

Precision agriculture: spatial and temporal variability of environmental quality. General reflections.

Ciba Foundation symposium·1997
Same journal

Uncertainty in hydrogeological modelling.

Ciba Foundation symposium·1997
Same journal

Optimal mapping of site-specific multivariate soil properties.

Ciba Foundation symposium·1997
Same journal

Modelling for precision weed management.

Ciba Foundation symposium·1997
Same journal

GIS support for precision agriculture: problems and possibilities.

Ciba Foundation symposium·1997
Same journal

Spatial sampling.

Ciba Foundation symposium·1997
See all related articles

Researchers elucidated tropoelastin structures across species, revealing conserved sequences with variations. Alternative splicing generates diverse elastin isoforms, indicating complex gene regulation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Elastin is a crucial protein for tissue elasticity.
  • Understanding elastin structure and gene regulation is vital for connective tissue research.

Purpose of the Study:

  • To determine the primary structure of tropoelastins from chicken, cow, rat, and human.
  • To investigate the structural conservation and variation in tropoelastins.
  • To explore the mechanisms of elastin gene expression regulation.

Main Methods:

  • Isolation and characterization of complementary DNAs (cDNAs) for elastin mRNA.
  • Sequence analysis of tropoelastins from multiple species.
  • Analysis of elastin gene structure, including introns and 5' flanking regions.

Related Experiment Videos

Main Results:

  • Elucidation of full-length tropoelastin primary structures, showing conserved domains and significant variations.
  • Identification of greater sequence differences between avian (chicken) and mammalian tropoelastins.
  • Discovery of extensive alternative splicing producing multiple tropoelastin isoforms from a single gene.
  • Characterization of the human elastin gene introns (Alu repeats, dinucleotide repeats) and 5' flanking region (G+C rich, multiple transcription start sites).

Conclusions:

  • Elastin structure is conserved but exhibits species-specific variations.
  • Alternative splicing is a key mechanism generating tropoelastin diversity.
  • Elastin gene expression is complex, regulated at multiple levels, involving intricate promoter elements and potential recombination sites.