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Related Experiment Videos

Development of biosensor for glucose estimation by ion sensitive field effect technique

J T V1, P R Vaya, M Singh

  • 1Biomedical Engineering Division, Indian Institute of Technology, Madras, India.

Indian Journal of Experimental Biology
|October 1, 1995
PubMed
Summary

Ion Sensitive Field Effect Devices (ISFED) show promise as biosensors. Researchers found silicon oxynitride offers improved pH response in Electrolyte-Insulator-Semiconductor devices for glucose estimation.

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Area of Science:

  • Materials Science
  • Biotechnology
  • Sensor Technology

Background:

  • Ion Sensitive Field Effect Devices (ISFED) are explored for biosensing applications.
  • Electrolyte-Insulator-Semiconductor (EIS) structures, specifically Electrolyte-SiO2-Si, are investigated for their response to pH variations.
  • The performance of silicon oxynitride as an insulator in EIS is compared to silicon dioxide (SiO2).

Purpose of the Study:

  • To understand the C-V characteristics and flat band voltage shifts in EIS structures concerning pH.
  • To evaluate the potential of EIS C-V characteristics as a biosensor for glucose estimation.
  • To investigate the immobilization of glucose oxidase for enhanced biosensing capabilities.

Main Methods:

  • Characterization of C-V (Capacitance-Voltage) curves and flat band voltage shifts in response to pH.

Related Experiment Videos

  • Fabrication and testing of EIS devices using silicon oxynitride and SiO2 as insulators.
  • Immobilization of glucose oxidase onto the EIS sensor surface.
  • Measurement of differential capacitance changes at a constant voltage in response to varying glucose concentrations.

    • Main Results:

    • Silicon oxynitride exhibits a superior pH response compared to SiO2 in EIS structures.
    • The differential capacitance of the glucose oxidase-immobilized EIS system shows a linear response to glucose concentration.
    • EIS C-V characteristics demonstrate potential for quantitative glucose estimation.

    Conclusions:

    • Silicon oxynitride-based EIS devices offer enhanced performance for pH sensing.
    • The developed EIS system utilizing immobilized glucose oxidase functions as a viable biosensor for glucose detection.
    • The linear relationship between differential capacitance and glucose concentration provides a reliable method for biosensing.