Recognition and specificity in protein tyrosine kinase-mediated signalling
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Updated: Sep 22, 2026

Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
1Division of Signal Transduction, Beth Israel Hospital, Boston, MA 02115, USA.
Abstract:
There are several factors that contribute to the specificities of protein tyrosine kinases (PTKs) in signal transduction pathways. While protein-protein interaction domains, such as the Src homology (SH2 and SH3) domains, regulate the cellular localization of PTKs and their substrates, the specificities of PTKs are ultimately determined by their catalytic domains. The use of peptide libraries has revealed the substrate specificities of SH2 domains and PTK catalytic domains, and has suggested cross-talk between these domains.
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