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Detection and isolation of nepoviruses on strawberry in the Czech Republic
1Department of Plant Virology, Academy of Sciences of the Czech Republic, Ceské Budĕjovice, Czech Republic.
Acta Virologica
|June 1, 1995
Abstract:
Arabis mosaic, strawberry latent ringspot, tomato black ring and raspberry ringspot nepoviruses were monitored using double sandwich enzyme-linked immunosorbent assay (DAS-ELISA) in 18 cultivars of strawberry Fragaria x ananassa Duch. in the Czech Republic. Arabis mosaic and strawberry latent ringspot viruses were detected, isolated and characterized on differential host plants and by electron microscopy. Both viruses were purified and antisera to them were prepared.