Related Experiment Video
Updated: Aug 15, 2026

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells
Published on: February 27, 2020
The uncoupling of Shc activation from distal intracellular signalling in a cell line which expresses a truncated
1Department of Medicine, Royal Melbourne Hospital, Parkville, Victoria, Australia.
Abstract:
We have examined epidermal growth factor (EGF) signalling in a CHO cell line (CHO11) which expresses a human EGF receptor truncated at amino acid 990. Previous studies showed that EGF treatment of these cells failed to increase prostaglandin production or phospholipase A2 activity. In the current study EGF increased the tyrosine phosphorylation of the intracellular signalling protein Shc in CHO11 cells but did not activate either of the downstream signalling enzymes raf or mitogen activated protein kinase (MAPK). The uncoupling of Shc activation from distal signalling in CHO11 cells contrasts with other cells which express similar mutant EGF receptors. The failure of She to activate distal signalling may reflect qualitative differences in the way that this protein is activated or could result from the activation of an inhibitory signalling pathway.
Related Concept Videos
Mitogens and the Cell Cycle
Receptor Downregulation in MVBs
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
Amplifying Signals via Enzymatic Cascade
Receptor Tyrosine Kinases
TGF - β Signaling Pathway
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

