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[Relation between cell proliferation and beta adrenergic receptor expression in activated T lymphocytes]
C Cazaux1, L Sterin-Borda, G Gorelik
1Centro de Estudios Farmacológicos y Botánicos (CEFYBO)-CONICET, Buenos Aires.
Acta Physiologica, Pharmacologica Et Therapeutica Latinoamericana : Organo De La Asociacion Latinoamericana De Ciencias Fisiologicas Y [De] La Asociacion Latinoamericana De Farmacologia
|January 1, 1995
Summary
Murine lymphocytes and a tumor cell line have fewer beta adrenergic receptors. The tumor cells lack functional receptors, suggesting alternative signaling pathways may be involved in cancer.
Area of Science:
- Immunology
- Pharmacology
- Cell Biology
Context:
- Beta adrenergic receptors (R) play crucial roles in immune cell function and are targets for neuroendocrine modulation.
- Understanding receptor expression and functionality in both normal and malignant lymphoid cells is essential for developing targeted therapies.
Purpose:
- To characterize beta adrenergic receptors on murine lymphocytes stimulated with concanavalin A (Con A) and the BW5147 tumor lymphoid cell line.
- To assess receptor functionality through hormone-dependent changes in cyclic adenosine monophosphate (cAMP) levels.
Summary:
- Radioligand binding assays using 125I-cyanopindolol (125I-CYP) revealed a reduced number of beta adrenergic receptors on both cell types.
- While lymphocytes showed a diminished response to beta-agonist stimulation, the BW5147 cell line was unresponsive, indicating absent functional beta R.
- Both cell types responded to Prostaglandin E1 (PGE1) with increased cAMP production, suggesting intact signaling pathways independent of beta R.
Impact:
- This study concludes that functional beta adrenergic receptors are absent in the BW5147 tumor lymphoid cell line.
- Findings suggest the potential involvement of alternative signaling pathways and neuroendocrine control mechanisms in tumor lymphoid cells.
- Results provide insights into the altered receptor expression and function in cancer, potentially informing future therapeutic strategies.