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Multiplication of VHS virus in insect cells
1Danish Veterinary Laboratory, Arhus N, Denmark.
Veterinary Research
|January 1, 1995
Summary
Insect cell cultures are susceptible to viral haemorrhagic septicaemia virus (VHSV), a pathogen affecting farmed rainbow trout. This VHSV, produced in insect cells, remained pathogenic to fish after cell culture passages.
Area of Science:
- Virology
- Aquaculture Pathology
- Cell Biology
Background:
- Viral haemorrhagic septicaemia virus (VHSV) is a significant rhabdovirus pathogen impacting farmed rainbow trout.
- Traditional production of recombinant proteins often utilizes insect cell cultures.
Purpose of the Study:
- To investigate the susceptibility of insect cell cultures to VHSV.
- To characterize VHSV multiplication and glycoprotein production in insect cells.
- To assess the pathogenicity of VHSV derived from insect cell cultures.
Main Methods:
- Infection of insect cell cultures with VHSV.
- Observation of cytopathic effects, including syncytia formation at specific pH.
- Analysis of VHSV glycoprotein size produced in insect cells compared to fish cells.
- Pathogenicity testing of cultured VHSV in rainbow trout.
Main Results:
- Insect cell cultures demonstrated susceptibility to VHSV infection.
- VHSV multiplication at pH 6.2 induced syncytia formation, comparable to baculovirus expression of VHSV glycoprotein.
- The VHSV G protein produced in insect cells was smaller than that from fish cells.
- VHSV propagated in insect cells retained pathogenicity to rainbow trout for at least two passages.
Conclusions:
- Insect cell cultures can support VHSV replication.
- Recombinant VHSV glycoprotein production in insect cells may yield altered protein characteristics.
- Insect cell-derived VHSV poses a continued risk to rainbow trout aquaculture.