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Updated: Aug 10, 2026

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
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Sensitive high-performance liquid chromatographic analysis of amlodipine in human plasma with amperometric detection
M Josefsson1, A L Zackrisson, B Norlander
1Department of Clinical Pharmacology, Faculty of Health Sciences, Linköping University, Sweden.
Abstract:
A narrow-bore HPLC assay with electrochemical detection for the determination of the calcium antagonist amlodipine in human plasma samples is presented. By using a single-step solid-phase extraction procedure on Bond Elut C2 columns, the sample preparation step has been considerably simplified and less time-consuming compared to earlier presented works. With a linear and reproducible calibration curve over the range 0.5-20 ng ml-1 plasma, the assay has successfully been used in the analysis of more than 500 plasma samples from a multicenter trial.
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