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A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium

Alessandra Argnani1, Rob J Leer1, Nicole van Luijk1

  • 1TNO Nutrition and Food Research Institute, Department of Molecular Genetics and Gene Technology, PO Box 5815, 2280 HV Rijswijk, The Netherlands.

Microbiology (Reading, England)
|January 1, 1996
PubMed

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In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...