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[In vitro studies on glycerol transformation by rumen microorganisms]

H Bergner1, C Kijora, Z Ceresnakova

  • 1Fachgebiet Tierernährung der Humboldt-Universität zu Berlin, Germany.

Archiv Fur Tierernahrung
|January 1, 1995
PubMed
Summary

Glycerol supplementation in sheep rumen fluid increases volatile fatty acids, particularly propionic acid. Most added 14C-glycerol was converted to propionic acid, with reduced bacterial nitrogen incorporation.

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Area of Science:

  • Rumen microbiology
  • Animal nutrition
  • Biochemistry

Context:

  • Rumen fermentation is crucial for ruminant digestion.
  • Glycerol utilization by rumen microbes is not fully understood.
  • Sheep are important livestock globally.

Purpose:

  • To investigate the effects of glycerol supplementation on rumen fermentation.
  • To determine glycerol utilization pathways in sheep rumen fluid.
  • To assess the impact of glycerol on volatile fatty acid production and microbial nitrogen.

Summary:

  • Sheep rumen fluid was incubated with varying glycerol concentrations (5-50%) and wheat starch.
  • Glycerol disappearance was rapid, dependent on concentration and time.
  • Volatile fatty acids, especially propionic acid, increased with glycerol addition and incubation time.

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  • 14C-glycerol was primarily incorporated into propionic acid, with minimal conversion to CO2.
  • Glycerol did not affect TCA-precipitable nitrogen but reduced 15N incorporation into bacterial nitrogen.

    • Impact:

    • Glycerol can be efficiently utilized by rumen microbes, increasing propionic acid production.
    • This suggests glycerol as a potential feed supplement to enhance energy availability in ruminants.
    • Understanding glycerol metabolism is key for optimizing ruminant diets and reducing methane emissions.