Evidence of a functional VIP receptor in cultured human retinal pigment epithelium

The effect of VIP on the intracellular cyclic AMP of human retinal pigment epithelium cultures has been studied. Functional VIP receptor has been demonstrated in cultures from eyes given by five normal donors (age 16-64) (N-HRPE). But it has been found to be absent from high passage number cultures obtained from a retinitis pigmentosa eye of an 84-year-old patient (RP-HRPE). After 3 min of reaction with 1 x 10(-6) M VIP, the intracellular cyclic AMP level has increased to 5-15-fold over the basal level. The maximal effect of VIP (20-fold over the basal level) has been observed at 1 x 10(-7) M VIP. The half maximal activity of VIP is 3-5 x 10(-8) M. The present study also demonstrates the inducibility of the VIP responsiveness in RP-HRPE cultures after they have been treated with butyrate. Curr. Eye Res. 14: 1009-1014, 1995.