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An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies
N L Anderson1, R Esquer-Blasco, J P Hofmann
1Large Scale Biology Corporation, Rockville, MD 20850-3338, USA. leigh@lsbc,com
Electrophoresis
|October 1, 1995
Abstract:
We have improved upon the reference two-dimensional (2-D) electrophoretic map of rat liver proteins originally published in 1991 (N. L. Anderson et al., Electrophoresis 1991, 12, 907-930). A total of 53 proteins (102 spots) are now identified, many by microsequencing. In most cases, spots cut from wet, Coomassie Blue stained 2-D gels were submitted to internal tryptic digestion [2], and individual peptides, separated by high-performance liquid chromatography (HPLC), were sequenced using a Perkin-Elmer 477A sequenator. Additional spots were identified using specific antibodies.