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Updated: Aug 1, 2026

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
Published on: January 28, 2021
Splanchnic production of discoidal plasma high-density lipoprotein in man
Abstract:
The morphology of human plasma high-density lipoprotein (H.D.L.) subfractions in arterial and hepatic venous blood from seven human subjects was investigated by electron microscopy. The H.D.L.3 subclass from both sources and the H.D.L.2 subclass from arterial blood were composed of spherical particles, identical with normal human peripheral venous H.D.L. In contrast, H.D.L.2 isolated from hepatic venous blood was composed of a mixture of spherical and discoidal particles. The latter tended to form rouleaux, and had a diameter similar to that of nascent H.D.L. particles obtained from the peripheral venous blood of a patient with lecithin: cholesterol acyltransferase deficiency. This supports the view that nascent H.D.L. is secreted into the splanchnic bed in man.
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