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Related Experiment Videos

Re-188 labelled antibodies

B A Rhodes1, C R Lambert, M J Marek

  • 1RhoMed Incorporated, Albuquerque, NM 87109-5802, USA.

Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine
|January 1, 1996
PubMed
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This study demonstrates a simple one-step kit for directly labeling monoclonal antibodies with Rhenium-188 (188Re). The resulting 188Re-labeled antibody showed high purity and tumor uptake, outperforming previous radioisotopes.

Area of Science:

  • Nuclear medicine
  • Radiopharmaceutical chemistry
  • Oncology

Background:

  • Monoclonal antibodies (mAbs) are crucial for targeted cancer therapy.
  • Direct radiolabeling of mAbs simplifies their preparation for radioimmunotherapy and radioimmaging.
  • Rhenium-188 (188Re) offers favorable characteristics for targeted radionuclide therapy.

Purpose of the Study:

  • To develop and optimize a simple, one-step kit for direct radiolabeling of monoclonal antibodies with 188Re.
  • To evaluate the radiochemical purity, immunoreactivity, and biodistribution of 188Re-labeled B72.3 antibody.
  • To compare the performance of 188Re-labeled B72.3 with antibodies labeled with other radioisotopes.

Main Methods:

  • Utilized a W-188/Re-188 generator eluate for labeling.

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  • Employed a pre-reduced lyophilized B72.3 antibody incubated with the generator eluate overnight.
  • Assessed radiochemical purity, immunoreactive fraction, and biodistribution in tumor-bearing nude mice.

    • Main Results:

    • Achieved a radiochemical purity of 88 +/- 7% and an immunoreactive fraction of 68.46 +/- 3.8%.
    • The immunoreactive fraction was higher than previously reported for B72.3 labeled with other radioisotopes.
    • Demonstrated comparable tumor uptake (7.9 +/- 2.4% injected dose/gram at 48 h) and biodistribution to 186Re and 111In labeled B72.3.

    Conclusions:

    • A simple, one-step kit for direct 188Re labeling of monoclonal antibodies is feasible and effective.
    • 188Re-labeled B72.3 exhibits excellent radiochemical properties and promising tumor targeting capabilities.
    • This method offers an advantageous alternative for preparing radiolabeled antibodies for targeted therapies.