Related Experiment Video
Updated: Aug 8, 2026

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs
Published on: December 27, 2016
Alcohol inhibits cell mitosis in G2-M phase in cell cycle in a human lymphocytes in vitro study
B S Ahluwalia1, L S Westney, S U Rajguru
1Department of Obstetrics and Gynecology, Howard University, College of Medicine, Washington, DC 20059, USA.
Abstract:
Alcohol abuse is associated with the loss of immunocompetence, which leads to decreasing resistance to infections. No single mechanism can be accountable for the detrimental effects of alcohol on the body's defense mechanism. We present data demonstrating that, in cultured lymphocytes, 10-40 mM alcohol in the media caused 18-90% decrease in cell mitosis (p < 0.001). There was a linear decrease in cell mitosis upto 40 mM alcohol; at 100 mM cell mitosis virtually ceased. This study aimed to determine in which phase of the cell cycle did alcohol mediate its effects. The results showed that DNA synthesis was not affected with up to 50 mM alcohol, suggesting that G1-S phase in the cell cycle remained unaffected. At 100 mM alcohol, DNA synthesis decreased significantly (p < 0.01). From the results of this study, we conclude that a subpharmacological dose level of alcohol (10 mM) significantly inhibited cell mitosis and the inhibitory effect of alcohol was mediated in the G2-M phase in the cell cycle. The G1-S phase was unaffected.
More Related Videos
12:02Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
08:33Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Published on: December 5, 2017
Related Concept Videos
Negative Regulator Molecules
Positive Regulator Molecules
Inhibition of Cdk Activity
Mitogens and the Cell Cycle
DNA Damage Can Stall the Cell Cycle
Inhibition of CDK Activity