[Ascorbic acid concentrations in ruminal fluid and in blood of cattle and sheep]

Plasma and ruminal fluid ascorbic acid levels were determined in Holstein calves aged four months and in ruminal fluid of merino rams aged 2,5 years under in vitro conditions. Ascorbic acid values decreased in plasma until 6th month. The highest Ascorbic acid value in ruminal fluid was found in 5th month. Under in vitro conditions shown ascorbic acid concentrations in ruminal fluid with time upward tendency. For analysis of ascorbic acid concentrations the acid phosphotungstate method were used for each samples.