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Related Experiment Videos

Burn injury alters coronary endothelial function

N M Garcia1, J W Horton

  • 1Department of Surgery, University of Texas Southwestern Medical Center, Dallas 75235-9031, USA.

The Journal of Surgical Research
|January 1, 1996
PubMed
Summary

Burn injury significantly impairs coronary endothelial function and nitric oxide production in rabbits. This disruption in coronary vascular reactivity may contribute to cardiac performance changes following thermal injury.

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Area of Science:

  • Cardiovascular Physiology
  • Burn Medicine
  • Endothelial Biology

Background:

  • Burn injuries can profoundly affect systemic hemodynamics and organ function.
  • Coronary endothelial function plays a critical role in maintaining cardiac performance.
  • The impact of thermal injury on coronary vascular reactivity and nitric oxide pathways requires further elucidation.

Purpose of the Study:

  • To investigate the effects of a significant scald burn on coronary endothelial function.
  • To assess changes in coronary vascular reactivity following burn injury.
  • To evaluate nitric oxide production, indicated by cyclic guanosine monophosphate (cGMP) levels, in burned versus sham-burned rabbits.

Main Methods:

  • Adult rabbits underwent a 30% total body surface area scald burn or sham procedure.

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  • Animals were resuscitated with Ringer's lactate solution.
  • Hearts were harvested at 2, 6, and 24 hours post-injury for ex vivo perfusion.
  • Coronary perfusion pressure (CPP) and coronary vascular resistance (CVR) were measured.
  • Endothelial function was assessed by measuring cGMP levels in coronary perfusate.

    • Main Results:

    • Coronary perfusion pressure (CPP) and coronary vascular resistance (CVR) decreased in burned rabbits compared to controls during the early postburn period, returning towards baseline by 24 hours.
    • Cyclic guanosine monophosphate (cGMP) levels in the coronary perfusate were significantly lower in burned hearts (27 +/- 1 fmole/ml) compared to sham-burned hearts (310 +/- 40 fmole/ml).
    • Reduced cGMP levels indicate impaired nitric oxide production by the coronary endothelium following burn injury.

    Conclusions:

    • Thermal injury disrupts coronary endothelial function, evidenced by reduced nitric oxide production.
    • Impaired endothelial function and altered coronary vascular reactivity are significant consequences of burn injury.
    • These findings suggest a mechanism contributing to postburn cardiac dysfunction.