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The 16S ribosomal RNA mutation database (16SMDB)

K L Triman1

  • 1Department of Biology, Franklin and Marshall College, Lancaster, PA 17604, USA.

Nucleic Acids Research
|January 1, 1996
PubMed
Summary

The 16S ribosomal RNA mutation database (16SMDB) catalogs mutations in Escherichia coli 16S ribosomal RNA. It details mutation phenotypes, detection methods, and literature for researchers studying bacterial genetics.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Bioinformatics

Background:

  • 16S ribosomal RNA (rRNA) is crucial for bacterial protein synthesis and is a common target for genetic studies.
  • Understanding mutations in 16S rRNA is vital for deciphering bacterial function and evolution.
  • Existing resources for cataloging these mutations were limited.

Purpose of the Study:

  • To create a centralized, accessible database of 16S ribosomal RNA mutations in Escherichia coli.
  • To provide comprehensive information on mutation identity, associated phenotypes, and experimental validation.
  • To facilitate research by linking mutations to relevant scientific literature.

Main Methods:

  • Compilation of mutation data from scientific literature.
  • Inclusion of information on mutation phenotypes (e.g., antibiotic resistance, growth defects).
  • Annotation of whether phenotypes were observed in vivo or in vitro.

Main Results:

  • The 16S ribosomal RNA mutation database (16SMDB) was established, listing specific mutated positions and alteration types in E. coli 16S rRNA.
  • Each entry includes phenotype descriptions, detection methods (in vivo/in vitro), and literature citations.
  • The database is accessible via FTP and the World Wide Web.

Conclusions:

  • The 16SMDB serves as a valuable resource for researchers investigating 16S rRNA genetics in Escherichia coli.
  • It consolidates critical mutation information, aiding in the study of bacterial molecular mechanisms.
  • The database promotes further research and discovery in microbial genetics and evolution.

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