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Interference of heparin in plasma levocarnitine determination with radioenzyme assay
A Marzo1, G Cardace, C Corbelletta
1Sigma-Tau S.p.A., Drug Metabolism and Pharmacokinetic Department, Pomezia, Italy.
Arzneimittel-Forschung
|December 1, 1995
Abstract:
This paper describes the interference of heparin (CAS 9005-49-6) in whole plasma used for free and total levocarnitine (L-carnitine, CAS 541-15-1) analysis. Alkaline hydrolysis required for total L-carnitine measurement does not overcome the problem. The same interference is also present in long-chain acyl-L-carnitine assay, because heparin precipitates together with proteins and long-chain esters of L-carnitine in the acid insoluble fraction of perchloric acid treated plasma sample. In this case an appropriate cleaning of the sample is recommended before the assay.