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Storage effects on dentin permeability and shear bond strengths
H E Goodis1, G W Marshall, J M White
1Department of Restorative Dentistry, University of California, San Francisco, USA.
Summary
Dentin permeability and bond strength are affected by how teeth are stored. Phosphate-buffered saline storage significantly decreases dentin permeability, potentially impacting dental material research results.
Area of Science:
- Dental Materials Science
- Biomaterials Research
- Dentin Properties
Background:
- Tooth storage solutions are crucial for preserving dental tissues.
- Standardized storage methods are essential for reliable dental materials research.
- Previous studies have not fully elucidated the impact of storage solutions on dentin properties.
Purpose of the Study:
- To investigate the effects of storage duration and solution type on dentin permeability.
- To evaluate the impact of storage conditions on dentin bond strength.
- To determine the suitability of common storage solutions for dental research.
Main Methods:
- Teeth were stored in five solutions: 70% ethanol, 10% formalin, distilled water, distilled water with thymol, or phosphate-buffered saline with thymol.
- Dentin permeability and lap shear bond strength were measured after 8 days, 15 days, and 6 months.
- A repeated measures design with n=20 was employed.
Main Results:
- Dentin permeability varied significantly with storage solution and duration.
- Phosphate-buffered saline storage led to a significant decrease in dentin permeability over time.
- Bond strengths were generally unaffected by storage duration or solution type, except for saline.
Conclusions:
- Storage conditions, particularly the use of phosphate-buffered saline, can alter dentin permeability.
- The observed changes in dentin permeability suggest that saline may be unsuitable for dental materials research.
- Careful selection of storage solutions is necessary to ensure the validity of research findings on dentin properties and bond strength.