[Evaluation of assay methods of glycohemoglobin: reference method and parameter]

Area of Science:

• Clinical Chemistry
• Analytical Biochemistry
• Diabetes Diagnostics

Background:

• Glycated hemoglobin (HbA1c) is a crucial biomarker for long-term glycemic control in diabetes management.
• Accurate measurement of HbA1c is essential for effective diabetes monitoring and treatment decisions.
• Various analytical procedures exist for quantifying glycated hemoglobin products.

Purpose of the Study:

• To present and evaluate the characteristics of analytical procedures for measuring glycated hemoglobin.
• To compare the performance of different methods for HbA1c determination.
• To recommend the most reliable method for clinical use.

Main Methods:

• Review and analysis of analytical procedures for glycated hemoglobin measurement.
• Performance evaluation of different techniques, including high-performance liquid chromatography (HPLC).
• Comparison of specificity and reliability of various assays.

Main Results:

• The study details the performance characteristics of different analytical methods for glycated hemoglobin.
• High-performance liquid chromatography (HPLC) demonstrates superior reliability and specificity for HbA1c measurement.
• Specific measurement of HbA1c is highlighted as a key factor in accurate diabetes assessment.

Conclusions:

• The authors recommend the specific measurement of HbA1c for clinical practice.
• High-performance liquid chromatography (HPLC) is identified as the most reliable analytical procedure for HbA1c quantification.
• Adoption of recommended methods ensures improved accuracy in diabetes patient monitoring.