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Expression of the rat MHC class I gene in the human B lymphoblastoid cell line Hmy 2 CIR

S K Salgar1, H W Kunz, T J Gill

  • 1Department of Pathology, University of Pittsburgh, School of Medicine, PA 15261, USA.

European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics
|December 1, 1995
PubMed

Rat MHC class I cDNA (e.g. RT1.A1) can be expressed optimally as a heterodimer consisting of the rat heavy chain and the human beta2-microglobulin in the human B lymphoblastoid cell line Hmy 2 CIR, which offers several advantages. Gene expression was detected by flow cytometry using anti-human beta2-microglobulin and anti-rat heavy chain antibodies.

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