Related Experiment Videos
Sigma s regulates pLS1 maintenance in stationary-phase Escherichia coli
M Espinosa-Urgel1, C Chamizo, A Tormo
1Departamento de Bioquímica y Biología Molecular I, Facultad de Ciencias Biológicas, Universidad Complutense, Madrid, Spain.
FEMS Microbiology Letters
|January 1, 1996
Abstract:
We have studied the influence of sigma s on the stability and number of copies of the promiscuous plasmid pLS1 in Escherichia coli. Our results indicate that pLS1 is less stable and has a lower number of copies in a rpoS mutant than in a wild-type strain during stationary phase. This behaviour does not seem to be due to differences in the expression of pLS1 replication regulators, but to be related to plasmid topology.