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On-column isomerization of sugars during high-performance liquid chromatography: analysis of the elution profile
T Nishikawa1, S Suzuki, H Kubo
1Department of Clinical Pathology, School of Medicine, Kitasato University, Kanagawa, Japan.
Journal of Chromatography. A
|January 12, 1996
Abstract:
Four monosaccharides (glucose, galactose, mannose and fructose) and one disaccharide (maltose) were subjected to high-performance liquid chromatography with UV or refractive index detection. Various profiles such as broad, tailed and splitted peaks were produced, depending on column temperature and eluent flow-rate because these saccharides underwent isomerization. In contrast, alpha-methylglucoside, a non-converting derivative, always produced a sharp peak. By analyzing these profiles kinetic constants of the isomerization were obtained and compared with the literature data.