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Related Experiment Videos

Multiple pathways for apoptotic nuclear fragmentation

L Dini1, S Coppola, M T Ruzittu

  • 1Dipartimento di Biologia, Università di Lecce, Italy.

Experimental Cell Research
|March 15, 1996
PubMed
Summary
This summary is machine-generated.

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Nuclear fragmentation during apoptosis occurs via multiple pathways, yielding diverse nuclear shapes. These distinct routes suggest a conserved physiological role for nuclear fragmentation in programmed cell death.

Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • Apoptotic nuclear fragmentation is a hallmark of programmed cell death.
  • The precise mechanisms and morphological variations of nuclear fragmentation are not fully elucidated.

Purpose of the Study:

  • To investigate the ultrastructural diversity of apoptotic nuclear fragmentation in U937 cells.
  • To identify alternative pathways leading to nuclear fragmentation during apoptosis.

Main Methods:

  • Analysis of U937 cell ultrastructure using electron microscopy.
  • Treatment of cells with various apoptogenic agents, including hydrogen peroxide and ADP-ribosylation inhibitors.
  • Assessment of actin involvement using cytochalasins.

Main Results:

Related Experiment Videos

  • Identified multiple distinct pathways for nuclear fragmentation, dependent on the apoptogenic inducer.
  • Observed actin-dependent chromatin budding into vesicles and nucleus splitting via internal cleavage.
  • Described a unique morphology under oxidative stress, potentially originating from mitotic cells.

    • Conclusions:

    • Apoptotic nuclear fragmentation is a complex process with multiple alternative pathways.
    • Morphological variations in nuclear fragmentation suggest convergent evolution and a significant physiological role.
    • The findings contribute to understanding the intricate mechanisms of programmed cell death.