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Cloning and characterization of mouse CCAAT binding factor
M A Hoeppner1, D J Gilbert, N G Copeland
1Department of Biochemistry, Northwestern University, Evanston, IL 60208 USA.
Nucleic Acids Research
|March 15, 1996
Summary
Two forms of mouse CCAAT binding factor (mCBF) are generated by alternative splicing. Their differing cellular localization suggests a novel regulatory mechanism for gene transcription in mammals.
Area of Science:
- Molecular Biology
- Gene Regulation
Background:
- CCAAT binding factor (CBF) is crucial for gene transcription.
- Understanding CBF regulation is key to deciphering cellular processes.
Purpose of the Study:
- To investigate the expression and function of mouse CCAAT binding factor (mCBF) isoforms.
- To elucidate the regulatory mechanisms of mCBF in mammalian cells.
Main Methods:
- Isolation of cDNA clones for mCBF.
- Analysis of adult mouse tissue RNAs.
- Overexpression studies in mammalian cells.
Main Results:
- Two distinct mCBF isoforms (mCBF1 and mCBF2) arise from alternative splicing.
- mCBF1 and mCBF2 mRNAs are ubiquitously expressed, with mCBF1 being more abundant.
- mCBF1 and mCBF2 proteins exhibit differential cellular localization (nucleus vs. cytoplasm).
- Co-expression affects protein localization, indicating a regulatory role.
Conclusions:
- Alternative splicing generates functionally distinct mCBF isoforms.
- Differential localization of mCBF isoforms provides a novel regulatory mechanism for gene transcription.
- The relative expression levels of mCBF isoforms can modulate CBF activity.