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Superoxide anion reduces the ability of myeloperoxidase to damage lipids
1Department of Forensic Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Japan.
Abstract:
The aim of this study was to determine whether lipids are damaged by myeloperoxidase (MP) not only with hydrogen peroxide (H2O2), but also with a H2O2-generating system. Using the hypoxanthine-xanthine oxidase-Cu,Zn-superoxide dismutase (HX-XO-SOD) system as an effective H2O2-generating system, we observed a reduction in the ability of MP to produce peroxylipids in the HX-XO-SOD-MP system, compared with the H2O2-MP system. We also noted that MP inhibited the production of monoepoxide by the H2O2-cytochrome c (Cyt c) system. These results suggest that MP plays a role as an absorber of active oxygen species and prevents phagocytes from self-destruction.