Gibberellin-repressible gene expression in the barley aleurone layer

Gibberellins are noted for their ability to induce expression of genes, such as alpha-amylase, in the aleurone layers of cereals. However, a number of mRNA species in the mature imbibed aleurone cell of barley, such as a storage globulin (Heck et al., Mol Gen Genet 239: 209-218 1993), are simultaneously and specifically repressed by gibberellin. In a continuing effort to understand this effect, we report cloning and characterization of two additional cDNAs from barley designated pHvGS-1 and pcHth3 that have high corresponding mRNA levels in the mature imbibed aleurone but are repressed 10-fold or more within 24 h of treatment with gibberellic acid (GA3). The extent of repression was concentration dependent and maximally effective at 10(-6) M GA3. Repression was also noted in the constitutive gibberellin response mutant, slender, in the absence of exogenous GA3. The antagonistic phytohormone, abscisic acid, had no effect or was weakly inductive of the steady-state levels of these mRNAs. During development of the seed, repressible mRNAs are present to different degrees in the maturing aleurone layer and embryo, but not in the starchy endosperm. Some repressible mRNA persists in the mature dry aleurone layer, but is degraded during imbibition, replenished by de novo transcription, and maintained at high steady-state levels until GA3 is perceived. Preliminary investigation suggests that repression is at least partly due to destabilization of the mRNAs which have estimated half-lives of 12 h or greater in the absence of GA3. pcHth3 encodes a member of the gamma-thionin gene family located on chromosome 7. pHvGS-1 corresponds to a gene on chromosome 3 of unknown function.