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Related Experiment Videos

Stromal cells regulate bcl-2 and bax expression in pro-B cells

L F Gibson1, D Piktel, R Narayanan

  • 1Department of Pediatrics, West Virginia University Health Sciences Center, Morgantown, USA.

Experimental Hematology
|April 1, 1996
PubMed
Summary

Bone marrow stromal cells are crucial for pro-B cell survival and proliferation by regulating bcl-2 expression. BAX expression correlates with apoptosis initiation in these developing B lymphocytes.

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Area of Science:

  • Hematology
  • Immunology
  • Cell Biology

Background:

  • B lymphocyte production is regulated by specific bone marrow microenvironments.
  • Fibroblastic stromal cells are key regulators of early B cell lineage development.
  • The precise roles of stromal cells in B cell viability, proliferation, and differentiation require further elucidation.

Purpose of the Study:

  • To investigate the role of bone marrow stromal cells in the survival and expansion of normal murine pro-B cells.
  • To determine if stromal cells regulate the expression of the proto-oncogene bcl-2 in pro-B cells.
  • To understand the relationship between bcl-2, BAX expression, and apoptosis in pro-B cells.

Main Methods:

  • Co-culture of pro-B cell clone C1.92 with stromal cell line S10.

Related Experiment Videos

  • Analysis of bcl-2 mRNA expression upon stromal cell removal.
  • Interruption of bcl-2 expression using antisense oligonucleotides.
  • Assessment of cell cycle progression and apoptosis.
  • Measurement of BAX expression levels.

    • Main Results:

    • Stromal cells are essential for the long-term proliferation and viability of pro-B cells.
    • Pro-B cells cultured with stromal cells express bcl-2; its downregulation coincides with apoptosis upon stromal cell removal.
    • Antisense inhibition of bcl-2 leads to G1 cell cycle arrest preceding apoptosis, while BAX upregulation directly correlates with apoptosis initiation.

    Conclusions:

    • Bone marrow stromal cells support pro-B cell survival and proliferation, partly through the regulation of bcl-2 expression.
    • bcl-2 may contribute to cell survival by modulating cell cycle progression in lymphopoietic cells.
    • Increased BAX expression is a strong indicator of apoptosis initiation in pro-B cells.