Immunocytochemical localization of acetylated alpha-tubulin in mouse resident peritoneal macrophages

Antibodies which recognize tubulin, actin and vimentin were used to localize microtubules, microfilaments and intermediate filaments, respectively, in whole Triton X-100 extracted macrophages. The cytoskeleton components were seen by transmission electron microscopy of cells labeled with secondary antibodies coupled to colloidal gold particles. All microtubules were labeled with a monoclonal antibody which recognizes alpha tubulin. In addition, few were labeled with an antibody recognizing acetylated alpha tubulin.