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Studies on shared antigenic/allergenic components among fungi
J Verma1, S Sridhara, B P Singh
1Centre for Biochemical Technology, Delhi, India.
Allergy
|October 1, 1995
Summary
Fungal allergens are common in India. This study found shared allergenic proteins, particularly a 45-kDa protein, among common fungi like Fusarium solani, crucial for diagnosing and treating fungal allergies.
Area of Science:
- Mycology
- Immunology
- Allergology
Background:
- Fungal allergens are prevalent aeroallergens in India.
- Understanding shared and unique fungal components is vital for diagnosing and treating fungal allergies.
Purpose of the Study:
- To characterize crude extracts of 11 common fungi.
- To identify shared antigenic and allergenic proteins among these fungi.
- To evaluate the allergenicity of Fusarium solani components.
Main Methods:
- Isoelectric focusing (IEF) and SDS-PAGE for protein separation.
- Immunoblotting with rabbit anti-Fusarium solani antibodies and pooled patient sera.
- Analysis of protein molecular mass and antigenicity.
Main Results:
- IEF revealed up to 46 protein bands; SDS-PAGE showed 18-40 bands (14-100 kDa).
- Shared antigenic proteins (14-100 kDa) were detected across fungi using rabbit antibodies.
- Significant shared allergenicity (14-70 kDa) was observed in patient IgE immunoblots, with a common 45-kDa protein.
Conclusions:
- Fusarium solani components share antigenic/allergenic proteins with other common fungi.
- A 45-kDa protein is a common allergen across multiple fungal species.
- These findings are critical for accurate fungal allergy diagnosis and immunotherapy.